Assessment of lymphocyte populations and their subsets in the patients with acute coronary syndrome

We examined 23 patients aged 40 to 65 years (mean age 54.526.72) with a diagnosis of acute coronary syndrome (ACS) at admission, who underwent emergency or delayed stenting day later. All had arterial hypertension as concomitant disease. Upon additional examination, blood troponin levels were determined, ECG was performed in the time dynamics. Acute myocardial infarction ST elevation diagnosed 7 patients, without elevation, 6 unstable angina rest, rest this group (Grace risk from 75 150 points, on average, 107.727.16 points). To assess immune status, especially, lymphocyte populations and subsets we used standardized techniques, i.e., flow cytometric assays Navios cytofluorimeter (Beckman Coulter, USA). The following subpopulations determined: CD45+ (panleukocyte marker for gating lymphocytes), CD45+, CD3+ (T cells), CD3+, CD4+ (helper inducers), CD8+ (cytotoxic T CD3+CD16+, CD56+ (TNK cells) CD3-, CD16+, (natural killer CD19+CD5+ (B CD4+, CD25+ (activated helpers, early activation phase), HLA-DR lymphocytes late phase). data obtained indicate that relative indices helper subpopulations, cells step, B increased syndrome, compared control group. At same time, there is trend increasing absolute values these indexes. subpopulation TNK proved be significantly both values, whereas percentages CD45+CD3+CD19- (p 0.01) cytotoxic 0.001) decreased showed terms. ratio CD4/CD8 almost doubled 0.001), due content T-helpers decrease lymphocytes. In clinical analyses ACS tendency leukocytosis shown, (10.155.22), shift band forms.